ChIP-Atlas: SRX4485113

Antigen

Cell type Class: Neural
Cell type: Hippocampus
MeSH Description: A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: ChIP-Seq: Hippocampi from 4.5 month old CK-p25 and age matched CK controls mice were isolated at 6 weeks after induction of p25 gene expression, and used for PU.1 ChIP-Seq. RNA-Seq: Hippocampi were isolated from the brains of mice at 3 and 4.5 months. Total RNA was extracted from FACS-purified CD11b+CD45 microglia using QIAGEN RNA Easy Mini Kit. ChIP-Seq: Libraries were prepared from ~1-5 ng ChIP (or input) DNA as described previously (Gjoneska et al., 2015) RNA-Seq: cDNA was prepared using SMART-Seq v4 Ultra Low Input RNA Kit and sequencing libraries were constructed using Nextera XT DNA library preparation kit.